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In some cases, aggregation of molecular systems is the focus of the experiment and the ability to quantify aggregation is of the utmost importance. TCSPC data collection was performed to up to numerical overflow of the photon counting board 65 photon peak count. The bottom panel of Figure 5 is the 10 ms binned photon trajectory with the HMM photon-by-photon state reconstruction overlaid. Blonde wife cum in mouth swallow. Creampie teen anal enema. For single-molecule measurements, the frequency-doubled laser light was transformed to circular polarization with a quarter-wave plate CVI Laser, model, QWPMR10 and focused to a diffraction-limited spot via a 60X, NA 1.